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KMID : 0881720100250020153
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Journal of Food Hygiene and Safety
2010 Volume.25 No. 2 p.153 ~ p.161
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Investigation of Norovirus Occurrence and Influence of Environmental Factors in Food Service Institutions of ChungCheong Area
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Jung Woo-Young
Eom Joon-Ho
Kim Byeong-Jo
Ju In-Sun
Kim Chang-Soo
Kim Mi-Ra
Byun Jung-A
Park You-Gyoung
Son Sang-Hyuck
Lee Eun-Mi
Jeong Rae-Seok
Na Mi-Ae
Yuk Dong-Yeon
Gang Ji-Yeon
Heo Ok-Sun
Yun Min-Ho
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Abstract
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The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with NH3-N, total solids and the consumption of KMnO4, out of such variables as NH3-N, total solids, the consumption of KMnO4, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson’s correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [NH3-N] + 0.023 [total solids] + 0.762 [consumption of KMnO4] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, R2 = 0.818, adjusted R2 = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were NH3-N, total solids and the consumption of KMnO4. In other words, when the measured values of NH3-N, total solids and the consumption of KMnO4 were higher, the possibility of appearance of norovirus increased.
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KEYWORD
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Norovirus, physicochemical factor, microbial factor, one-step RT-PCR, semi-nested PCR
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